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It showed that 24 h after transfection we could observe presence of both endogenous and exogenous β-actin mRNA, whereas γ-actin mRNA was present almost only in exogenous form (Fig. 2c, d).
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It can be due to the fact, that half-life of actin proteins is usually 2 3 days, so 1 day after transfection part (β-actin) or the entire (γ-actin) pool of mRNA is already in the exogenous form, while at the same time we can still observe in the cell endogenous actins as proteins (Antecol et al. 1986; Cheever et al. 2011; Dugina et al. 2009).
To test this possibility, hydrogen peroxide (H2O2) was used as an exogenous form of ROS in a range of concentration used in previous reports.
In koalas, vertical transmission of the exogenous form of KoRV does not occur via the germ line67.
Porcine endogenous retroviruses (PERVs) represent remnants of an exogenous form that have become integrated in the domestic pig (Sus scrofa) genome.
In some experiments, endothelial cell proliferation was measured upon exposure to CM from young and senescent HPMCs enriched in exogenous recombinant forms of human VEGF, GRO-1/CXCL1, IL-8/CXCL8, and MCP-1/CCL2 as well as in specific neutralizing antibodies against those factors.
In some experiments, endothelial cell migration was measured upon exposure to CM from young and senescent HPMCs enriched in exogenous recombinant forms of human VEGF, GRO-1/CXCL1, IL-8/CXCL8, and MCP-1/CCL2 as well as in specific neutralizing antibodies against those factors.
In the present study we used a paradigm in which endogenous and exogenous forms of attention are orthogonally manipulated in order to investigate their relation with conscious perception within the same design.
WT transfectants expressed both endogenous and exogenous forms of PXN at the same level as untransfected cells, but PXN expression was doubled in all three mutants.
Consequently, the tendency of the central Mg to form coordination bonds with electron pairs in exogenous ligands, a reflection of its Lewis acid properties, is increased.
The data clearly demonstrated that exogenous formaldehyde-DNA adducts form in a highly non-linear fashion, with a 21.7-fold increase in exposure (i.e., 0.7 to 15.2ppm) causing a 286-fold increase in exogenous adducts.
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