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To facilitate analysis of the structures, non-adherent cells incorporated in excess medium were removed from each well prior to quantitative analysis.
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After cells had settled in the wells, most of the excess medium was removed, a cover glass added, and the sandwich tightly sealed.
The embryos were then quickly placed onto a Cryotop (Kitazato Supply Co., Fujinomiya, Japan), followed by aspiration of excess medium with a fine pipette and quick immersion in liquid nitrogen.
nitrogen excess medium.
Excess medium was discarded and the cells were air-dried.
At the beginning of the batch phase, glucose was present in excess in the medium, leading to the production of ethanol as a main metabolite in addition to biomass (Crabtree effect, overflow metabolism).
Most of the discriminated elementary flux modes are typical routes known to be active in cultured mammalian cells, while excluded routes are related with the catabolism of those amino acids not in excess in the medium, thus being directly used for protein synthesis.
At its height, in excess of 100 medium-slow meteors per hour might be seen under the best conditions.
If UNICEF should receive funds in excess of the medium-term funding requirements for this emergency, UNICEF will use those funds to support other, underfunded emergencies.
Cells were cultured in a non-hypoxic environment, with all nutrients in excess in the culture medium.
Oxygen, ammonia and other important inorganic metabolites are assumed to be in excess in the growth medium.
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