The photoanode was then rinsed in ethanol to remove excessive dyes and dried in air.
When precipitated in ethanol, to remove unbound ligand species, and then dried, CHAT contained 31 ± 1% copper (w/w) by ICP-OES analysis.
The prepared ZnO nanostructured electrodes were immersed in an ethanol solution containing 0.5 mM of N719 dye (cisbis isothiocyanato) bis (2,2′-bipyridyl-4,4′-dicarboxylic 2,2′-bipyridyl-4,4′-dicarboxylic 2,2′-bipyridyl-4,4′-dicarboxylic 2,2′-bipyridyl-4,4′-dicarboxylicdye acidruthenium IIlly and drying in air.
The photocathode was prepared by immersing a NiCo2O4 sample in an ethanol solution containing 0.5 mM of N719 dye (cis-bis isothiocyanato bis(2,2′-bipyridyl-4,4′-dicis-bis isothiocyanato bisII)) (Solaronix SA, Aubonne, Switzerland) for 3 h, followed by rinsing in ethanol to remove dye absorbed physically, and drying in air.
After annealing at 300°C for 30 min, zinc oxide photoanodes were sensitized in a 0.5 mM solution of RuL2 NCS 2, L = 2,2'-bibyridyl-4,4'-dicarboxylic 2,2'-bibyridyl-4,4'-dicarboxylic 2,2'-bibyridyl-4,4'-dicarboxylic 2,2'-bibyridyl-4,4'-dicarboxylice immersed in ethacid to remove excess dyes.
All particles were washed in ethanol to remove endotoxins, and then dried in a dessicator [ 6].
In the next step, the nucleic acids were collected by centrifugation at 14,000xg for 15 min. The pellet was washed in 80% ethanol to remove salt and redissolved in 1 ml of TE.
The samples were then placed in an ether bath and subsequently were immersed in 100% ethanol to remove the ether.
Then, they were dipped into 0.05 M Cd(NO3 2, ethanol solution, rinsed in pure ethanol to remove excess of the precursor and dried in air.
SA molecules were chemisorbed on the ZnO nanoflake surfaces by immersing the sample (reaction time of 90 min) in an ethanol solution of 8 mMol SA (C18H36O2) for 24 h, followed by rinsing it in absolute ethanol to remove excess reactants, and then dried naturally.
After staining, the tissue was incubated in 70% ethanol to remove the chlorophyll.
