The labeled cDNAs obtained from the two experimental conditions (i.e. infected and non-infected samples) were mixed in equivalent quantities (pmols) of incorporated Cy3 and Cy5 in order to obtain a final volume of 20 μl hybridization solution.
A volume of buffer 2× (urea 7 M, thiourea 2 M, chaps 1.5%, ASB-14 1.5%, destreak reagent 12.5 μl.ml-1, DTT 10 mM, ampholyte 1%) was added in equivalent quantities to the previously pooled samples.
A commentary printed immediately following the paper made the point that the large volumes of blood and fresh frozen plasma, given in equivalent quantities to both groups, would have amounted to over 300g albumin; the 25 75g supplement to the protocol group could not be expected to have any additional pharmacological effect.
The benefits of these improved properties on cytotoxicity were demonstrated in a set of experiments in which equivalent quantities of purified NTR enzyme were incubated with V79 cells in the presence of varying concentrations of CB1954.
The scope of this study is limited to substitutions of an equivalent quantity in grams.
In order to understand the role of mild chronic cerebral hypoperfusion in time-related Aβ deposition, we investigated the fate of peripherally applied human Aβ1-42 peptides (25 ng; the equivalent quantity found in one week in the serum of littermate APP/PS1 transgenic mouse) in the brain capillaries of hypoperfused (24 hours after 1 VO) mice.
While the presence of equivalent quantities in a sample of both Mon 810 and Mon 88017, for example, could be due to the presence of the same quantity of two different products, this situation would be suggestive of the presence of a stacked-trait product.
All other cells received an equivalent quantity of DMSO in the presence or absence of TGF-β (5 ng/ml).
After being unable to find a suitable housekeeping gene for normalising the data, it was decided to express the real-time data relative to the equivalent quantity of RNA in the reaction, given that the total RNA concentrations had been normalised before cDNA synthesis.
While these end-point PCR amplifications are not quantitative, the fluorescence from ethidium bromide (EtBr) indicated that in those tissues where both 900- and 1000-bp frareents amplifiedfied, these molecules are likely to be in equivalent molar quantity within the genome.
Envisioning the day when we may all have fuel cell cars, General Electric researchers have built a prototype that makes the equivalent quantity of hydrogen: plug it in, and it splits water molecules to generate one kilogram per hour of hydrogen.
