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Species within each plot were planted in equal densities and equally spaced but randomly placed within the plot.
For this purpose, cells were seeded in equal densities in 24 well-plates (see above) and harvested by trypsinization.
The resultant nymphs of each replicate per line combination were distributed in equal densities across separate 5 L containers with ad libitum access to food and water.
(B ) Representative images of cortical neurons cultured for 14 days in vitro (DIV), either alone or in equal densities with thalamic neurons, with or without hevin treatment.
The fish were cultured in equal densities in circulating tanks under a natural light cycle, and fed to satiation with a stage-specific commercial diet for juvenile fish (1 4CDX and Masu 5 8p from Nippon Formula Feed Mfg Co., Ltd) until the end of the experiment (about 6 months).
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Cells were seeded at low density (5×103 cells/cm2), cultivated for 5 days and then replated in equal density and cultivated for 5 days more.
For cell culture experiments, HET-1A cells were plated in equal density in 24-well plates and supernatants were collected at 18 h following treatment.
Cells were harvested and seeded at equal densities in triplicate in 96-well plates.
Prior to the experiment, the juvenile salmon were raised under equal densities in 100-L tanks and then pooled together in 1800-L tatks at densities resembling that of the farmed environment (see Fraser et al. 2010 for details).
Cells were seeded at equal densities in 6-well plates and, after reaching confluence, they were washed and incubated for one additional hour in the same medium (control) or medium supplemented with γ-irradiated LPS purified from E. coli serotype 055∶B5 (Sigma-Aldrich) or mouse recombinant tumor necrosis factor-α (TNF-α, 410-MT; R&D Systems).
RAW 264.7 cells (American Type Culture Collection, Manassas, VA, USA) and HC-11/R1 cells were plated at equal densities in six-well tissue culture plates and allowed to grow for 48 hours in complete HC-11/R1 media.
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