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Both annexin-V-labelled and unlabelled fractions were transferred into fresh media for use in ensuing experiments, which were carried out immediately to avoid loss of viability of the annexin-V-positive fraction.
The 50% inhibitory concentration (IC50) of ADI in PANC-1 was determined to be 1 mU/mL at 72 h and was used as the treatment dose in ensuing experiments.
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Instrumental background was measured in a separate set of experiments and automatically corrected for in the ensuing experiments, according to the manufacturer's instructions.
To verify whether this implied a bias in the ensuing experiments, clinical grade of OA and baseline serum cytokine levels were compared between this group of patients and the eight patients from whom no SF could be aspirated.
As a framework for the ensuing experiments described in this study, these connections are diagrammed in Figure 1; this figure shows both known connections between IL-6 and other molecular players (e.g., Ca2+ and TLR4) as well previously unappreciated links (e.g., ganglioside involvement in IL-6 activation that acts even in the absence of SMF, offering a new controlling mechanism for IL-6).
The ensuing experiments resulted in successful chelation of AlF into peptide conjugated with p-SCN-Bn-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA).
As stated in the method section of the manuscript, animal instrumentation and ensuing experiments were approved by the institutional review board for animal research (project 04585-04 evaluated par the "Ethical committee Anses-Enva-UPEC").
We do understand that ensuing experiments attempt to verify the effect shown in Figure 2B but readers may get confused here.
As the role of cytosolic SLPI was the focus of ensuing experiments, we also confirmed the presence of SLPI in cytosolic fractions of CF and COPD cells).
Subsequently we used this marker combination to characterize most of the ensuing experiments.
When the cells were confluent, they were divided again and used for the ensuing experiments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com