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Homogenous dispersion of OMMT in EMB matrix was observed.
The decline in EMB treatment efficacy for this purpose is an important issue for salmon producers around the world, and particularly for those in the Atlantic Ocean where widespread EMB tolerance in sea lice is recognized as a significant problem.
Histological analysis revealed active caspase-3 in EMB specimens.
In emb 1738 plants, two-thirds of the embryos are arrested at the early transition to heart stage [ 22].
Levels of activated caspase-3 in EMB samples were determined using ELISA, specific for the activated protein form (Invitrogen, Paisley, UK).
Histological heart graft rejections will be documented by the presence of damaged myocytes (or cellular necrosis) in EMB (former grade 2, grade 2R and grade 3R).
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The M306V mutation in the transmembrane protein embB is one of the most frequently observed mutations in EMB-resistant strains [32], putatively preventing ethambutol from interfering with biosynthesis of the arabinogalactan layer in the cell wall.
Eleven isolates had double embB mutations in EMB-resistant isolates.
In embR, nonsynonym mutations occurred at P49A, S104N, and P243S in EMB-resistant isolates and at L125S and R230W in EMB-susceptible isolates.
Nonsynonym mutations occurred at S244T, I179T, E149D, and A38T in EMB-resistant isolates and none in EMB-susceptible isolates in ubiA.
Polygenic resistance is likely in EMB-resistant L. salmonis (Espedal et al. 2013).
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