> -wrap-foot> Six calves were experimentally infected with virulent BRSV, either passaged in vivo (BRSV-Snk, n = 3, calves A1-3), or in vitro (BRSV-Dk, n = 3, calves B1-3).
Cells are seeded onto a suspended permeable membrane, and under sterile culture conditions, cells proliferate freely in "growth conditions" and are either passaged at 80% confluence or allowed to form a fully confluent monolayer.
Short term testing was performed in rat by creating arterial access from the medial tail artery and performing the Extroducer transvascular passage in either the subclavian or carotid artery testing two different stages; the transvascular passage and the deployment of a tip.
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To test whether the accelerated growth of NSs requires continued exposure to the HMF, primary NSs exposed to the GMF or HMF for 7 days were passaged in either the GMF or the HMF, giving four experimental groups: GMF to GMF (G-to-G), GMF to HMF (G-to-H), HMF to GMF (H-to-G), and HMF to HMF (H-to-H) (Fig. 2A).
The expanded cell populations were detached from flasks using 1 × Trypsin/EDTA solution, in preparation for either passaging or xenograft injection.
Cells transformed with oncogenes in vitro invariably acquire additional genetic abnormalities, either during passage in vitro or subsequently as an adaptation to growth in vivo.
have been excluded from large portions of historically accessible habitats in the western United States, either by passage barriers or by large-scale changes in habitat quality.
As participants either in the passage of the Telecommunications Act of 1996 or in decisions on whether to update the Act, we write to provide our unique insight into the meaning and intent of the law.
The procyclin mutants described to date [14], [20], [21], [24] were generated in laboratory-adapted stocks that had either been passaged in rodents or maintained in culture for an extended period of time.
Conversely, SA-β-Gal activity measured with the fluorescence method rose in proportion to the calendar age of the donor either in early-passage cultures or in primary cell isolates from omental tissue.
