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Moreover, these limitations are further exacerbated in ecological experiments if participants are free to move, requiring substantial manual interventions to correct head motion artefacts9,13.
In ecological experiments, live plants can also be imaged in small microcosms with mid-IR transmitting ZnSe windows.
To use transformed plants and reverse genetics in ecological experiments to measure the consequences of gene silencing on plant performance, we suggest the adoption of the following procedures: A reasonable guess about phenotypic effects of gene silencing can be made when a particular gene with an annotation is silenced.
The stable co-expression of silencing inhibitors in N. benthamiana and N. tabacum plants resulted in much higher transgene expression levels, but both plant species suffered from abnormal growth and altered leaf morphologies, which would invalidate their use in ecological experiments [ 68, 108].
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The objective of this study was to evaluate the effect of transformation, based on tissue culturing and A. tumefaciens infection, for possible side-effects on ecologically relevant performance traits, in order to determine how many and which controls are needed in functional ecological experiments with gene-silenced N. attenuata plants.
Obviously, the above hypotheses, based solely on bioinformatic analysis of transcriptome sequences, need further investigations, in particular ecological experiments and functional protein assays, to functionally link the proposed candidate genes to the observed traits.
In this system, ecological experiments have shown that the current dominance of exotic species is not due to competitive superiority, but a result of habitat fragmentation and changes in herbivory and disturbance regimes since European settlement.
To this end, we use a variety of approaches -- from ecological experiments in the field to molecular and developmental genetics in the laboratory.
WITHIN the bright-blue and green walls of Emilio Carranza, a three-classroom primary school in a rural part of the state of Puebla, a lesson is engrossed in a book of "Ecological Experiments and Facts".
We summarize some of the metrics used to compare the performance of monocultures and polycultures and describe how they are used to evaluate the degree of competition versus complementarity in both agricultural and ecological experiments.
The timescale in which biological or ecological experiments can be run precludes classic "invasion biology" from assessing the long-term effects thousands of years or more of modern human-mediated introductions.
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