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To estimate the distributed electrical current image in the brain at each time sample we used the Minimum Norm Approach47 on an average brain as implemented in BESA Research 6.0.
The advantage of GPC and other linear MBPC methods is in garanteed convergence within each time sample, but they are not able to deal with strong process nonlinearities.
As cMEM sources consisted in non-linear estimates for each time sample independently, it was not obvious that it would reconstruct temporally smooth time courses.
After three washes in PBS for 5 min each time, samples were incubated with Histofine simple stain MAX-PO (M)(Nichirei, Tokyo) for 30 min at room temperature, according to the manufacturer's instructions.
In each time, one sample was selected and used as the test set.
FST values compare differentiation between lakes in each of the time samples and with all samples pooled together.
On the basis of sampling size calculations, 15 juveniles were collected for each species at each time of sampling in each nursery.
Each group in each time point contained six samples.
The individual SAA measurements are shown in dot plots representing each time point sampled in Figure 2. Hp levels in the control group were low throughout the sampling period with a mean value of 0.11 ± 0.01 mg/ml, and the mean level in the scrapie group up until 22 weeks of age was similar at 0.10 ± 0.02 mg/ml.
The ordering of the transitions is shuffled in each time block in order to sample a different set of signaling rates within the networks.
The composts were also turned and mixed in the composters each time after sampling to prevent shortage of oxygen pore required for aerobic composting.
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