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See individual Figures for actual dosage tested in each separate experiment.
After remapping, we then evaluated the presence of differentially expressed genes (DEGs) between AD and controls in each separate experiment.
To ensure the performance of Ki67 and M30 staining, cytospins of MDA-MB231 and SKBR3 staurosporine-treated cells were included in each separate experiment as positive controls.
The average number of colonies in DMSO-treated controls was considered 100% clonogenic survival in each separate cell line and in each separate experiment.
Negative controls, prepared by omitting the corresponding primary antibody and adding the secondary immunoglobulin G (IgG) isotype antibody, were also used in each separate experiment.
In the same way and in each separate experiment a medium blank and corresponding spikes were prepared and used as negative and positive controls.
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A 10 μM (maximal) isoprenaline concentration was included in 2 3 wells in each plate for each separate experiment for H-cAMP accumulation to allow agonist responses to be expressed as a percentage of the isoprenaline maximum for each experiment.
A 10 µM (maximal) isoprenaline concentration was included in each plate for each separate experiment for CRE-SPAP production and 3H-cAMP accumulation, to allow agonist responses to be expressed as a percentage of the isoprenaline maximum for each experiment.
In order to graphically display data of three independent experiments in one combined graph, data of each separate experiment were normalised to the mean of the respective control samples.
A total of 20 embryos were examined for each condition in each separate replicate experiment.
Spiked HepG2 were included as controls in each separate immunofluorescence experiment performed for patient samples.
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