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Concurrent positive control animals are only necessary during validation, but positive control DNA must be included in each plating.
Degradation reactions of the Mg substrate in each plating stage are considered to figure out how to suppress degradation of substrate and to achieve uniform deposition of a plating layer.
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In each plate, endogenous control, samples and negative controls were analyzed in triplicate.
To determine the extent of bacterial colonization, live embryos in each plate were euthanized.
Two polymethylmethacrylate plates were used as substrates and a microchannel was carved in each plate.
The comb plates of the smaller ctenophores used in this work contain fewer cilia, but even here many thousands of cilia must be incorporated in each plate.
Each of the 96 samples in each plate were quantified using Qubit® and 25 ng of each sample used to compile a library.
The distance of the orifice rows (h) equals D corresponding to the orifice in each plate respectively.
Internal positive- and negative-controls were included in each plate.
Aspergillus terreus (from stock plate) was added in each plate for growth.
Cups (10 mm in diameter) were made in each plate using a sterile cork borer.
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