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In each passage, cells were infected with the passaged viruses (∼MOI of 0.1).
With periodically refreshing the culture medium, a high expansion fold of 60 77 was achieved in each passage.
During the PRV infection, the CRISPR-Cas9 system was efficient in cleaving the UL30 gene in each passage.
The cells cultured on rLN511E8 became confluent within 8 10 days after plating (the average fold change was 132 in each passage (Figure S2B)).
In each passage, it's the barbed payoff line that says a lot about Coe's true opinion of his charismatic rival's popularity.
With serial passaging, suspension cultures in spinner flasks were able to expand mES cells as aggregates for 12.5-fold in each passage of 4 days.
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To plot viability curves, dilution factors were factored in at each passage to report cumulative live cell numbers relative to sgAAVS1.
We simulated the two ends and the middle of this plagiarism severity gradient by using or neglecting the above plagiarism-avoidance behaviors in six rewritten versions of each passage in the PKS (Table 1).
As expected in these conditions, we observed a progressive decrease in mammosphere formation at each passage, due to exhaustion of adult stem cells (Cicalese et al, 2009).
Testing of the supernatant and the remaining cell layer in the infected CHSE-214 cells before each passage resulted in Ct values slightly above 20 (AHRV-7 assay).
Cells were passaged every 7 8 days by dissociation of bulk neurospheres with 0.05% trypsin/EDTA (Gibco) and cryopreserved at each passage in a Good Manufacturing Practice facility.
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