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Figure 9 shows the average occupancy of the McRAITs installed in each marker in the pipeline.
In each marker result, the yield-positive allele of the genes was shown with a green letter.
Doing so allowed us to characterise in detail the allelic dropout in each marker, and to establish a set of criteria to minimise artefacts and maximise data fidelity.
Rather than automatically assuming a worst-case scenario, we measured the level of dropout directly in each marker and relied on a less stringent multiple tubes approach, more comparable to protocols suggested elsewhere e.g., [17], [21].
In each marker, the average number of bands scored varied from 7 16.
The frequency of methylation in each marker was consistently higher in LOH-negative tumors than in LOH-positive tumors.
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With HincII, markers were covered across almost every sample, while in RsaI each marker was covered in only ~30% of samples.
One specific and important recent development in this area is the use of combinatorial marker assays in which each marker is targeted with a probe that is labeled with two or more fluorescent tags.
CM utilises all marker haplotype information, whereas RM utilises information contained in each individual marker and all possible marker pairs but not in higher order interactions.
Subtle variance in coronal height of each marker in the fracture fragment will further improve the analysis performance.
To determine whether there is preferential pairing behaviour within Q165 we examined linkage in repulsion of each marker with all other markers in the map.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com