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In each determination, 25 drop tests were made to calculate the H50.
In each determination, a minimum of 200 cells was evaluated.
Approximately 800 μg protein was used in each determination and the absorption of zinc ion was recorded at a wavelength of 213.9 nm.
At least 200 macrophages were counted in each determination, and those containing three or more internalized particles were considered positive for phagocytosis [ 25, 26].
A positive control consisting of pooled sera from mice immunised with HER-2/neu ECD-Fc fusion protein was included in each determination.
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Each determination in a single sample was performed three times.
Different number of technical replicates was used in each analytical determination (see specific technique for details).
The highly purified p1.1eGFP plasmid was used as a quantity calibrator using five different concentrations for each determination performed in triplicate.
Each determination was done in duplicate.
Each determination was performed in triplicate.
Each determination was carried out in triplicate.
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