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Appropriate positive and negative controls were included in each assay.
Triplicates were conducted in each assay of the parameters.
The mean number of live insects for each treatment in each assay was then calculated.
A standard antibacterial agent (amikacin 30 mcg) was included in each assay as positive control.
Numbers in parentheses indicate the number of cells observed in each assay.
Each CPC concentration was replicated four times in each assay (replicates are connected by grey lines).
Each mutant was analyzed in duplicate in each assay, and each experiment was performed in triplicate.
Uninfected McCoy cell cDNA and water as template were used as negative controls in each assay.
Equal amounts of protein were pooled and analyzed in each assay.
The quantitative data were obtained from 4 to 5 independent assays with duplication in each assay.
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Samples of extract equivalent to 104 cells were used in each assay.
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