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Each qPCR reaction was performed in duplicates with at least three biological replicates.
The experiments were performed in duplicates with a n = 6 each.
Shake flask fermentation was conducted in duplicates with the hydrolysates (Table 2) under same conditions as previously described.
Serum samples were analyzed in duplicates with Luminex Plate in a 1 2 dilution and per manufacturer´s directions.
The same observer who measured all CMR data in duplicates with an interval of >3 months separated from each other determined the intra-observer reproducibility of the analysis.
Experiments A, B, and C were performed in duplicates with 2 M NaCl and 1.5 M CaCl2 at 25, 50, or 80 °C, respectively.
Cells were treated in duplicates with 67/natGa-DOTATOC, 67/natGa-DOTATATE or 67/natGa-NODAGA-JR11 67/natGa-NODAGA-JR11 67/natGa-NODAGA-JR11r 1 h at 25 °C.
ELISAs were performed in duplicates with commercial kits (BD Bioscience) according to manufacturer's instructions.
All reactions were run in duplicates with the mean quantity taken.
The genotype frequencies were all in Hardy-Weinberg equilibrium (P>0.05) and 96 samples (4%) were run in duplicates with a 100% concordance rate.
Real-time qPCR was performed in duplicates with the Light Cycler480 instrument (Roche Diagnostics) using the Roche's SYBR-Green mastermix and prevalidated primer pairs (Qiagen).
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