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Quality assessment was performed in duplicated for each eligible study by two independent reviewers (Chen and Huang) using operationalized prognostic biomarker reporting guidelines [ 9] and extract details on 16 items (Additional file 1: Table S1).
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TLC detection was performed in duplicate for all samples.
The lipid content was measured in duplicate for each sample.
Experiments were performed in duplicates for three times.
Measurements were made in duplicate for each analysis.
All the experiments were performed in duplicate for each dose.
The measurements were carried out in duplicate for each sample.
The drying process was performed in duplicate for each drying method.
Samples were taken every 2 h and analysed in duplicate for size distribution.
Three independent experiments were performed in duplicate for real-time PCR.
At AnaMar AB, all the samples were tested in duplicate for COMP and CILP-2.
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