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Cytokines were assayed in duplicate and variation (co-efficient of variance, CV) between replicates determined.
All samples were performed in duplicate and variation between duplicates was <10% for every reported value.
All assays were performed in duplicate (coefficient of variation = 0.01).
Immunoblotting experiments were conducted in duplicate, with no variation in results observed.
All assays were performed in duplicate (coefficient of variation = 0.01).
Immunoblot experiments were conducted in duplicate, with no variation in results observed.
Samples and standards were analyzed in duplicate and only variation coefficients <15% were accepted.
Reactions were performed in duplicate and CT variation between them was < 1.5.
Each experiment was carried out in duplicate and no variation was obtained among them.
Each sample was measured in duplicate; intra-assay variation among the duplicates for all samples was <10%.
Using conventional Taq DNA polymerase (Qiagen), 99 cDNA samples were amplified in duplicate to reduce variation due to saturation effects.
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