Sentence examples similar to in duplicate to the volume of from inspiring English sources

Each sample was analyzed in duplicate with the volume of a single reaction added up to 15 µl which contained 20 ng template and a final primer concentration of 0.6 µM.

All samples and positive controls were assayed in duplicate to minimise effects of random variations in volumes due to pipetting errors.

Each sample was prepared in duplicate to contain 2×10 cells and 67 nM of a test antibody in a final volume of 200 μL.

All of the controls, standards and samples were added in duplicate at a volume of 100 µL/well.

Each reaction was run in duplicate in a volume of 25 μL in low 96-well white PCR plates sealed with optical flat 8-cap strips (Bio-Rad Laboratories, USA).

Each reaction was done in duplicate in a volume of 20 µL with 96-well optical-grade PCR plates, sealed with optical sealing tape (Bio-Rad).

Female plasma samples, nestling plasma samples and the chloroform extracted yolk fraction were diluted 1∶1,000 in sample buffer and added, in duplicate, at a volume of 100 µL/well.

All qRT-PCR reactions were performed in duplicates in a total volume of 25 μl containing Power SYBR Green Master Mix Applied Biosystemss) and 250 or 500 nM of each primer (depending on reaction efficiency).

DNA methyltransferase assays were carried out at 37°C for the indicated time in duplicate with a total volume of 25 μL of reaction mix, as described previously [ 4].

Reactions were plated in duplicate in a total volume of 25  μL using concentrations of 200 and 100 nmol/L of the corresponding primers and probe, respectively.

Each amplification reaction was performed in duplicate in a final volume of 20 μL containing 2 μL of the cDNA.