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Protein expression profiles were generated from 20 pairs, in duplicate, of normal mucosa and Dukes' C colorectal cancers.
This strategy allowed us to generate 12 relative activity values (in duplicate) of the same variant, which are then averaged to give the final value.
Fifty aliquots of 220 μL (i.e., the volume to perform the NS1 RDT in duplicate) of this NS1 control serum were prepared and stored at −80°C.
Sequence analysis (in duplicate) of these samples was performed using the forward primer only, since the reverse primer p695 is closely flanking the mutation hotspot.
Absolute quantification was checked by amplification, in duplicate, of cDNA synthesized from Tempus™ total RNA (whole blood samples) from three different animals of each species.
Three biological samples in duplicate of quantitative PCR experiments were performed for each sample as described in García-Rojas et al. [ 57].
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IVR fermentation measurements were performed in duplicate on each of six samples from 2005.
These chromosome regions have undergone further duplications in the teleost fish tetraploidization, 3R, resulting in duplicates of both the Hox clusters and the NPY and PYY genes.
Harris and Lasky predicted the birth of a chain, with eating while clapping becoming a hot ticket in duplicates of the Herts & Tallant theater in Boston, Chicago and elsewhere.
To determine kinetic parameters, initial rates of hydrolysis were obtained from five time points (0 16 min) and 14 p NPX concentrations (0.25 80 mM) using 7.8 nM BXA43 at the above conditions (in duplicates of four independent reactions).
All assays were run in duplicates of two independent experiments and mean values are reported.
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