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All assays in this study were performed in duplicate format, and data showed reproducible and concordant results.
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qPCR reactions are run in at least duplicate format.
All experiments were performed in duplicate (6-well format) or triplicate (24-well format) and repeated three times.
Paper data collection forms will be delivered to the data management unit (DMU) on a daily basis where they will be entered in duplicate into electronic format.
Samples were plated in 96 well format in duplicate after each of conjugated VEGF-1/HRP polyclonal secondary antibody was added.
‡mNT for MERS-CoV was done in a microtiter plate format in duplicate at dilutions 1 80 and 1 800.
For each chip, normalisation was applied to the left and right sides separately (spatial positioning of clones spotted in duplicate was in the format of two grids located on the left and right side of each array when orientated horizontally).
All reactions were performed in duplicate in 96-well plate format.
The array format was in duplicate cores per patient.
HEK293, Hela, NIH-3T3 cells were purchased from ATCC and maintained in high glucose (4.5 g/l) DMEM supplemented with 2 mM glutamine, 10% FBS, and 2 mM Penicillin/Streptomycin. Transfections to HEK293, NIH-3T3 and Hela cells were performed with Lipofectamine 2000 (Invitrogen) in duplicate in 24-well plate formats when cells are at 70 80% confluency.
These strains were arrayed in duplicate on fifty-one 384-format plates using a colony picker.
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