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Similar controls were obtained from a Saanen goat and included in duplicate for testing goat sera.
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Each measurement in a given sample was performed in duplicate for both tested and endogenous genes, and the threshold cycle (Ct) –the fractional number at which the amount of amplified target reached a fixed threshold– was determined.
Each chronic wound site was sampled in duplicate for parallel testing.
The microarray experiments were performed in triplicate for the three sequenced strains, E. coli MG1655; EDL933; and Sakia and at least in duplicate for the test strains.
SEM experiments were carried out in duplicate for each strain tested, and representative images of biofilms were selected.
The ChIP-PCR results were included in Supplementary Table 1, and the PCR primers used in ChIP assay and their sequences are listed in Supplementary Table 2. PCR was conducted in duplicate for each experimental condition tested.
At AnaMar AB, all the samples were tested in duplicate for COMP and CILP-2.
At IBEX, all the samples were tested in duplicate for all assays (C2C, CPII, C2C-HUSA and CTX-II and urine creatinine).
After incubation, supernatants were recovered by centrifugation (800 g, 10 min), and 40 µl was tested (in duplicate) for the presence of morphine using a morphine-specific ELISA kit (see below).
All conditions were tested in duplicate for each independent measurement.
Three independent transfections were carried out in duplicate for each of the concentrations tested.
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