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Standards of human HGF were prepared at concentrations of 3 2000 pg/mL in duplicate for construction of the standard curve.
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TLC detection was performed in duplicate for all samples.
The lipid content was measured in duplicate for each sample.
Measurements were made in duplicate for each analysis.
All the experiments were performed in duplicate for each dose.
The measurements were carried out in duplicate for each sample.
Cultures were made in duplicate for each hippocampus.
Each sample was plated in duplicate for each time point.
Each assay was performed in duplicate for all cell lines.
Experiments were performed in duplicate for each patient.
All samples were measured in duplicate for both assays.
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