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Studies of paleopolyploid plants have demonstrated that functional categories generally associated with higher network connectivity tend to be retained in duplicate following whole genome duplication (WGD) [ 28].
Samples were added in duplicate, following incubation and washing, an ascending concentration of the NaSCN (0 7M) was added to the wells.
All the determinations were evaluated in duplicate following best practice in clinical analysis.
To substantiate the genomic data and further identify proteins contributing to the apoptotic resistance phenotype, B-SWCNT and B-Control cell protein lysates were subjected to a human apoptosis protein expression array (see Additional file 1: Table S1 for array layout) in duplicate following manufacturer's instructions (R & D Systems, Minneapolis, MN, USA).
Tumor extracts containing 5 µg of total protein were analyzed/well in duplicate following manufacturer's instructions.
The TaqMan™ PCR was conducted in duplicate following standard protocols using the ABI PRISM 7700 (Applied Biosystems, Darmstadt, Germany).
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The samples were analyzed in duplicates following manufacturer's instruction.
Rapid divergence in gene expression profiles of duplicates following the duplication event results in expression profiles as diverse as those of singletons.
qRT-PCR reactions using RNAs extracted from TVs collected in each time point were performed in duplicate in following program: 50 °C × 1, 5 minutes; (95 °C × 1, 20 seconds; 95 °C, 30 seconds and 60 °C, 30 seconds) × 40 cycles.
The water-retention field capacity of each biochar sample was measured in duplicate gravimetrically following the previously established protocol (Kinney et al. 2012).
WT and isogenic Rad21+/− ES cells were seeded in 35 mm plates at appropriate densities in duplicate plates following a single dose of graded γ-ray irradiation (0, 2, 4, 6 and 8Gy).
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