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This confers the possibility to create asymmetric assemblies with different components bound to only one side of a membrane or to embed transmembrane structures in distinct orientations.
It is possible that the ring structure of proline might allow the substrate to be recognised in a different orientation within the PAT2 binding site or even in distinct orientations (as both an α- and a δ-imino acid) which might help explain why both d-proline and trans-4-hydroxy l-proline have similar affinities for PAT2 to that observed with l-proline [26,27,37,38].
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Notably, the modeled orientation of 1 bound to CalU16 or CalU19 appears to be different from 1 with CalC consistent with prior observations that the late stage biosynthetic intermediates en route to 1 bind the glycosyltransferases involved in 1 maturation via distinct orientations.
Staining of f-actin using Alexa Fluor-conjugated phalloidin revealed that the abdominal wall contains musculature in four distinct orientations: alary muscles, intrasegmental lateral muscles, intersegmental muscle bands, and transverse body muscles (Figures 5, 6).
These modelling experiments suggested that each protein pair could potentially interact in two distinct orientations, which involved mainly hydrophobic interactions between the α2/α5 surface (the aforementioned hydrophobic patch) in one DED and α1/α4 surface of the adjacent DED.
Third, the two PR70 EF-hand calcium-binding motifs adopt distinct orientations in the PR70 holoenzyme, which provides possible explanations for the effects of calcium binding on holoenzyme function.
Secondly, the presence of elongated structural features allowed us to dock our molecular model in a distinct orientation (cross correlation coefficient 0.865).
Such differences in accounting for action imply distinct orientations that make investigating the transition or intersection between certain problematizations (e.g., trade-off and paradox) and approaches (e.g., compromise and synergy) worthwhile.
Yet the different backbone positions of the Gln side chain in addition to neighbors that are differently conserved in MnSODs versus FeSODs can produce distinct orientations and different proximity to the OH /H2O ligand.
At one level similar to trade, medicine in these parts tended to have two distinct orientations: Country medicine, and European medicine.
Two distinct orientations and positions were found in the asymmetric unit.
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