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The impulse load delivered by a single shot generates enough kinetic energy in disrupted liquid material to do significant damage to surrounding solid structures.
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Tissues were delivered in RNAlater stablilization reagent (QIAGEN, cat. no. 76104) and were disrupted in liquid nitrogen before nucleic acids isolation.
Potato tissues were disrupted in liquid nitrogen using mortar and pestle.
All tissues were collected in RNAlater stabilization reagent (Qiagen. cat. no. 76104), transported on dry ice and stored at −80 ° C. The solid tissues were disrupted in liquid nitrogen prior to RNA extraction.
The tissue was disrupted under liquid nitrogen in a mortar and pestle then RNA extracted using TRIzol reagent (Invitrogen) according to manufacturer's instructions.
In conventional two fluid nozzles, the high velocity air imparts its energy to the liquid and disrupts the liquid sheet into droplets.
Fatty acids were extracted from frozen cell pellets (15 to 20 mg) previously disrupted with liquid nitrogen and glass beads in a homogenizer (15 seconds, maximum speed, Retsch Mixer Mill MM400).
Cells were harvested after 24 h of induction by quick centrifugation and immediately frozen in liquid nitrogen, disrupted with glass beads in a bead-beater and total RNA was extracted using the RNeasy Mini kit (QIAGEN), including the on-column DNAse treatment.
Pools of six male flies, aged 7 days, were frozen in liquid nitrogen, disrupted with a pestle in 75 μl PBS with 0.1% Tween-20 (PBS-T) containing Complete Protease Inhibitor (Roche) and lysed by adding 25 μl of 4 × sodium dodecyl sulphate sample buffer, 5-min incubation at 95°C and sonication for 5 min at low power in a Bioruptor (Diagenode).
Briefly, samples snap-frozen in liquid nitrogen were disrupted in tissue lyser II (Retsch, Haan, Germany).
Briefly, samples frozen in liquid nitrogen were disrupted in tissue lyser II (Retsch).
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