Here, LD based association analysis showed strong associations of multiple SNPs in different candidate HKT genes with the salt tolerance traits.
Three different measures of genetic distance were used: FST, Dest and a measure of allelic dissimilarity, along with a direct assessment of the total number of alleles in different candidate networks.
In conclusion, our study demonstrates that EcoTILLING can be successfully employed in B. vulgaris to survey a large panel of plant accessions for allelic variants in different candidate genes.
In order to model and test for interactions between SNPs in different candidate genes, standard logistic regression analysis was performed using the statistical software package R (The R Project for Statistical Computing, [ 17]).
In short, a sign of a recurrent mutation (that is, alleles of not too high frequency, seen in different candidate families) would support a risk related role of this locus, but it is not seen.
Similarly, other significant associations located in different candidate genes, such as sucrose synthase (SUSY), Cellulase (KOR), and Cinnamoyl-CoA reductase (CCR) (Table 5), were also supported by studies finding that they were involved in lignocellulosic cell wall development.
The acceptor medium was selected by determining the saturation concentration of vitamin E acetate in different candidate receptor solutions, selecting the one that provided the highest solubility, that is, 24.4 mg/mL.
Then, having done that, they skew the race in favor of different candidates in a naked display of their ability to influence the outcome.
In McCutcheon v. Federal Election Commission, Shaun McCutcheon, a political activist in Alabama, contributed to 16 different candidates in federal races in 2011-12.
