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In developing this protocol to amplify the EIV eight-segments, a series of processes, including phylogenetic analysis based on different influenza hosts, in silico analyses to estimate primer specificity, coverage, and variation scores, and investigation of host-specific amino acids, were progressively conducted to reduce or eliminate the negative factors that might affect PCR amplification.
We observed the recommendations of the PRISMA statement [ 12] in developing this protocol and review conduct.
We discuss the scope for applying realist evaluation and the methodological challenges we encountered in developing this protocol.
In developing this protocol, various types of culture media were investigated to identify the optimal growth conditions.
We thank Mrs Gemma Tur, Project manager at FISABIO (Fundación para el Fomento de la Investigaciónn Sanitaria y Biomédica de la Comunidad Valenciana) who has helped the research team in developing this protocol.
In developing this protocol, the following recommendations were taken into consideration: 1) the effective features of a life story intervention design for nursing home residents; and 2) suggestions on the best format for compiling life history resources for older people living in institutional settings.
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We would also like to thank Richard Bergenstal, MD, (USA), Frank Snoek, PhD, (Netherlands), and David Owens, MD, (UK) for their expert advice and assistance in developing the protocol and this manuscript.
In order to systematically develop this protocol, the results of spectral power distribution, radiation profile, and radiant power measurements of a variety of UV-LEDs are presented.
However, we understand that Microsoft won't be involved in developing the protocol, just the financial funding.
All team members were involved in developing the protocol.
Both had been participants in developing the protocol.
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