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The alcoholic PVP-I2 complex is indicated in delimitation of the surgical site.
These steps were conducted independently for individuals employed in delimitation analyses, and for the reduced set used in dating analyses.
Notably, it has allowed for quantification of uncertainty in delimitation of species [ 32] and avoids the use of non-independent pairwise sequence distances (e.g. in [ 23]) as data.
Molecular systematic studies of plants have frequently depended on chloroplast DNA (cpDNA) sequences as a primary source of data for investigations in delimitation of high-order taxonomic groups (for reviews, see Olmstead and Palmer, 1994; Petit et al., 2005).
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Although single mitochondrial genes such as COI used in DNA barcoding have proved highly effective in species delimitation in animals and plants [ 1, 2], it is important test hypotheses of putative species with independent nuclear loci.
Traditionally, ecological factors have been considered as being extremely important in species delimitation leading, in some cases, to a large number of taxa being proposed in some genera, including Acarospora sens.
In contrast, species delimitation in Archaea and Bacteria is likely to remain to be ultimately based on the 70% similarity threshold in DNA-DNA reassociation, which was established to mimic phenotype-based species concepts in Enterobacteria [80] and has recently be successfully emulated itself by the comparison of genome sequences [81], [82].
In addition, species delimitation in Clade I-B (species with long rod sclerites, cylindrical autozooids and smooth axes) remains unclear.
In addition, the use of multiple loci in species delimitation has increased rapidly over the last few years and are currently being applied to a large number of taxonomic problems [ 2– 6].
Particularly in high hazard areas, our work could help in spatial delimitation policies, urban planning and development of plans for the protection of human lives and assets.
Modelling approaches can aid in species delimitation only if the species studied actually diverge in their response to the environmental variables incorporated in the analysis [31].
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