Exact(23)
These results indicate that NSCs can be generated in defined conditions from hESCs adapted to defined conditions.
In fact, in order to derive "pure tumor" cell lines in defined conditions, all earlier approaches took measures to minimize the "contamination" of cultures with TME-elements.
We therefore tested whether NSCs grown to passages 15 25 in defined conditions could differentiate into dopaminergic neurons in PA6-CM.
As seen in Fig. 2A, NSCs propagated in defined conditions for 15 passages expressed Sox1 and nestin, and differentiated into β-III-tubulin+ neurons, GFAP+ astrocytes and GalC+ oligodendrocytes in conditions promoting differentiation (Fig. 2B C).
We have previously shown that hESCs can be maintained in defined conditions for prolonged time periods while retaining their pluripotency, and our current experiments suggested that each of the remaining steps could utilize such a line to generate dopaminergic neurons.
In pursuing this goal, we have developed a GMP-compliant scalable process of generating neural stem cells (NSCs) and further differentiate them into dopaminergic neurons in defined conditions from hESCs adapted to a defined medium.
Similar(37)
The observation that Δ40p53 may exert, in defined condition and cell context, a positive effect on p53 transcriptional activity, may account for some of the biological characteristics of animal models expressing this isoform.
It has been shown that factorial design of experiment approach is very helpful in defining conditions for an optimum performance in such complex systems.
Such designs represent the first step in defining conditions that will optimize, or perhaps even control, plant growth and development.
The main result of the analysis of model (1 - 2) consists in defining conditions required for maximum reduction of the tumor load.
Human iPSCs were cultured in chemically defined conditions in Essential 8 medium (Life Technologies) and grown on recombinant human vitronectin-coated surfaces (VTN-N, Life Technologies).
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