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Sections (5 µm) of formalin-fixed paraffin-embedded muscles were dewaxed with xylene and hydrated in decreasing concentrations of ethanol.
Fixed liver tissue was embedded in paraffin, cut into 5 µm thick sections, deparaffinized in xylene, and serially dehydrated in decreasing concentrations of ethanol.
Slides were immersed in 3 changes of xylene for 10 minutes each to remove the wax, followed by rehydration in decreasing concentrations of alcohol, before submersion in buffer.
Sections (6 µm) of formalin-fixed paraffin-embedded BAT were dewaxed in xylene, rehydrated in decreasing concentrations of ethanol and treated with 3% H2O2 (Sigma) in absolute methanol for 10 min at RT to quench endogenous peroxidase activity.
The same tissue block used for H&E histopathology was used to cut sections for immunohistochemistry. Formalin-fixed thyroid sections were deparaffinized in xylene, rehydrated in decreasing concentrations of ethanol, and washed in phosphate buffer saline (PBS).
For immunohistochemical staining slides were "deparafined" by incubation at 60°C for 30 minutes followed by immersion in xylene then in decreasing concentrations of ethanol (100, 95, 70%) as previously described [24], [25].
Tissue sections were deparaffinised in xylene and rehydrated using ethanol in decreasing concentrations.
Paraffin-embedded sections (5 μm thick) were dewaxed and rehydrated in decreasing concentrations of ethanol.
Tissues were deparaffinized in xylene and rehydrated in decreasing concentrations of ethanol and distilled water.
Adjacent sections were deparaffinised in xylene and rehydrated in decreasing concentrations of ethanol.
Sections were then immersed in xylene, followed by rehydration in decreasing concentrations of ethanol.
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