Exact(7)
Recovery experiments in CSFs show recoveries between 97 and 102%.
The interannual change in CSFs was large (1.7 2.5-fold 1.7 2.5-folddy), depending mainly on the variations oforathisent river distudyge (e.g. high flow year vs low flow year).
Table 5 shows the relationship of holding size to the number of cases in CSFS holdings after the index case was removed from the case count.
Adenylate kinase assay in CSFs was carried out in isotonic buffer A containing 3 mM Mg2+, 2.5 mM levamisol, 0.1 mM dipiridamol, 0.1 mM suramine (buffer A) and 2 mM ADP as substrate.
However the increase of susceptible genotypes to atypical scrapie and the increase in the number of atypical cases confirmed in CSFS holdings, due to the statutory Initial Cull and the re-stocking with Type 1 and 2 sheep, do not necessarily imply a significant increase of the overall prevalence of atypical scrapie in the national flock.
Nucleotidases assay in CSFs was carried out in incubation mixture composed of: isotonic buffer A with 3 mM Mg2+ or 2mMM Ca2+, 2.5 mM levamisol (alkaline phosphatase inhibitor) [ 33], 0.1 mM dipiridamol (adenosine deaminase inhibitor) [ 34], and 1 or 2 mM nucleotide (ATP, ADP, AMP) as substrate.
Similar(53)
Results: 92% of patients (56/61) had positive PCR in CSF and in 48% (29/61) enterovirus was isolated in CSF.
VZV serology and PCR in CSF were not performed.
PMNs in CSF did not present enhanced markers of activation.
In CSF [12], this set directly identifies the activated tags.
No causative bacteria could be found in CSF.
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