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However, the greatly extended shelf life was mainly ascribed to the inhibited microbial growth in CSB.
As for the preservatives application in CSB, fewer researches were conducted than that of fresh noodles.
Comparing with noodles, few researches on the hydrocolloids application in CSB were reported so far.
Here, we designed and synthesized 24 splice switching oligonucleotides (SSOs) to skip exon 3 in CSB mRNA.
Interestingly, however, short-term 2-AAF exposure induced lacZ mutant levels in Csb mice almost as high as those found in Xpa or Xpc mice.
The HMFR + T depicted a higher ASB (p < 0.01; 65% vs 35%) than the LMFR + T, without differences in CSB between HMFR + T and LMFR + T.
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To avoid possible artifacts due to CSB TAP overexpression, we chose a stably transfected clonal cell line, whose CSB expression level was grossly similar to the endogenous CSB level observed in CSB-proficient MRC5 cells (data not shown).
A similar trend for CSB was also observed in CSB-suppressed cells albeit much reduced due to suppression of CSB by shRNA.
ChIP assay also revealed the presence of CSB at the neurod1 promoter site in CSB-proficient cells and the recruitment of CSB was further enriched at the promoter site during/after differentiation.
Impaired neuritogenesis coupled with the reduced expression of MAP2 at mRNA and protein levels observed in CSB-suppressed cells prompted us to investigate the possibility that CSB had a role in the transcriptional regulation of MAP2 either alone or in combination with other transcription and chromatin remodeling proteins.
Consistent with reduced MAP2 expression in CSB-depleted neural cells, tandem affinity purification and chromatin immunoprecipitation studies revealed a potential role for CSB in the assembly of transcription complex on MAP2 promoter.
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