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The pH of the solution was adjusted by adding HNO3 (0.1 N) and NaOH (0.1 N) as required in course of the experiments.
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Furthermore, 1 animal in the L1 overexpressing SENA group, 2 animals in the wild-type SENA group, and 2 animals in the PBS group died in the course of the experiments and were thus excluded.
The estimates for effective population size both support the consistency in the fly cultivation techniques employed in the course of the experiments and corroborate the effective population size estimated from the experimental procedures.
Recent STR analysis has been performed within 6 months before the beginning or in the course of the experiments for all cell lines.
In the course of the experiments, we have found the importance of chip cleanliness, voltage polarity and the external measuring circuit.
In the course of the experiments, the anodic biofilm response and energy production efficiency have been investigated by experimental design approach, taking substrate and fresh inoculum – mesophilic anaerobic sludge (MAS) – addition into account as factors.
This developmental stage has been used to avoid any bending artifact of the roots in the course of the experiments.
To assure that the maximum soma areas were precisely recorded, the focal plane was continuously adjusted in the course of the experiments.
No foxes were injured in the course of the experiments.
Daily clinical evaluation of joints was performed in the course of the experiments.
Although cysTMT methodology is able to identify and quantify potential redox-regulated proteins, it does not address the issue of protein turnover in the course of the experiments (Supplementary Figure S2).
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