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Gene regulation was assessed in coronal sections from three rostrocaudal levels ('rostral', approximately at +1.6 mm relative to bregma (Paxinos and Watson, 1998); 'middle', +0.4; and 'caudal', −0.8) in a total of 18 sectors representing the caudate-putamen and five sectors representing the nucleus accumbens.
In coronal sections, glutamate puffs performed at a distance from the recorded cells usually evoked inhibitory responses, except when the recorded neuron was adjacent to the external capsule, in which case excitatory responses could be evoked from ejection sites along the external capsule.
Stathmin1 cells were counted in coronal sections.
The difference was evident in coronal sections taken from different parts of the dLGN.
Immunohistochemistry revealed broad expression of cGKII (Fig. 2E) and HCN2 (Fig. 2F) in coronal sections of mouse brain.
Next we examined the developmental expression of SPIG1 in coronal sections from E11.5 to P0 by in situ hybridization.
All the measurements were performed in coronal sections through the midbrain and the distances were measured in pixels using ImageJ.
Animals were then perfused with a fixative solution and brains were removed and sliced in coronal sections.
Next, we studied the calvarial bones in coronal sections of E18.5 pRb-null and control littermate skulls.
Quantifications of immunoreactive cells were performed by fluorescence microscopy blindly in coronal sections of three intact and three lesioned hemispheres.
The optical density of pCREB-immunoreactive cells was measured in the cortical plate in coronal sections (+2.16 to −0.36 mm from the bregma).
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