Fig. 2 Chondrogenic differentiation capacity of TECs. a Alcian blue staining of a TEC in control medium or in chondrogenic differentiation medium.
SAAD potentiated the cytotoxicity of H4IIE cells by arsenic or mercury (i.e. EC50, 19 and 5 μM in SAAD vs. 401 and 42 μM in control medium, respectively).
In selected experiments HYAFF sheets seeded with SMCs were wrapped to obtain cylindrical shape and then cultured in control medium or SA additioned medium for up to 28 days.
After 7 days, TAS-embedded gels in control medium reached SSM (1.45 ± 0.12 kPa) near normal pancreas, while gels maintained with conditioned medium achieved higher SSM (3.38 ± 0.146 kPa) consistent with tumors.
Undifferentiated hMSCs were maintained in control medium.
We compared melanoma cells in control medium, 200 mM methyl sulfone and 200 mM urea.
LNCs were cultured as single-cell suspensions, as described previously [10] with 2.5 μg/ml ConA or in control medium.
Briefly, groups of mice were intranasally infected with 25 µl of appropriate dilutions of the virus stock in control medium.
In ectoderm explants grown in control medium we observed the appearance of numerous hair follicles (arrows in Fig. 5A).
Similarly, WFA induced higher levels of apoptosis in endothelial cells grown in STS-CM than in control medium (Figure 6B).
HUVEC were seeded into 96-well plates coated with 1% gelatin at a density of 5×103/well and cultured in control medium for 24 hours before experiments.
