In contrast to the incorporation of dATP opposite M1dG or dG, both the Km and kcat were different for incorporation of dTTP and dGTP opposite to M1dG or dG.
This is in contrast to SO4 incorporation, which was enhanced with 100 or 1000 n M CNP in the presence and absence of IL-1β (P < 0.001).
In contrast to thymidine incorporation assays, the CFSE proliferation assay is more informative since, with the use of flow cytometry it allows the identification of proliferating cells by specific surface markers.
From the results it is evident that, in contrast to our expectation, incorporation of (4R -FPro into EGFP, which should favor the trans conformation of 9 Pro bonds, interferes with a correct protein folding, whereas (4R -FPro wintoits oppositEGFPfect obviously does not.
Another explanation relates to the NMR assay, which, in contrast to a radioactive incorporation assay, focuses exclusively on the detection of the predominant product.
The results of our study revealed that, in contrast to compound 8' o', incorporation of further double bond did not enhance the activity.
In contrast to single-nucleotide incorporation assays, analysis of full-length extension products in the presence of all four dNTPs can provide a measure of truncated, untargeted (i.e., random), mutations and/or frameshifted extension products.
Importantly, in contrast to the irreversible incorporation of PPL into the CE layer of epidermis through covalent crosslinking by TGases, the accumulation of PPL at the cellular periphery of hepatocytes occurred in a reversible manner, suggesting a different mode of usage for PPL in cutaneous and non-cutaneous tissues.
In contrast to heterotrophs, phytoplankton 13C incorporation was not affected by attachment and in fact exhibited lower cell-specific incorporation at higher temperatures.
In contrast to the 10 12% BrdU incorporation of skin γδ T cells in 6-week old lean db/+ mice, only half as many γδ T cells incorporated BrdU in db/db mice (Figure 2E, right panel, and 2F).
