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At the early stage of construction, the LEBCs in vitro develop without internal limitations in constructs in the bioreactor.
In addition, incorporating heparin and NGF promoted the adhesion and viability of iPS cells in constructs.
Instability of eukaryotic DNA in constructs propagated in prokaryotic hosts is a frequently observed phenomenon.
Thornes et al. [37] and Klitzman et al. [17] did not have any failures in constructs in either group.
Surface TATVHL peptide was effective in promoting the quantity of cartilaginous components in constructs after dynamic cultivation.
Our objective is to quantify the mechanical properties of the forming ET phase in constructs that utilize NNW scaffolds.
At 4 weeks, the numbers of cells, DNA and collagen II were greater in constructs engineered by fresh cells.
Subcutaneous implantation of hydrogel cultures showed greatly improved neocartilage formation in constructs loaded with TGF-β1 compared with controls.
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The PCR generated knock-in constructs were transformed into AAC null yeast (URA-AAC2).
Knock-in constructs were designed with inserts at the Xba I site at the Rosa26 locus as indicated in Figure 1A.
Our goal in determining the XCI status of the 74 knock-in constructs was to characterize cis-acting elements involved in the spread of epigenetic silencing.
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