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However, the main limitation of the PCR-based methods, including LM-PCR, is the incapability in construction of reference database, especially containing strain patterns of isolates from different laboratories.
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The LMS method (used for the first time to construct nationally representative growth references of Polish school-aged children and adolescents) has certain advantages in dealing with skewed data in construction of growth references.
In total, 70 326 VH gene sequences were used in construction of the human HD1 reference sequence database.
Open image in new window Fig. 3 Construction of reference arc.
An adaptive method of parameter estimation is therefore proposed in this article, which includes the construction of reference function and the two-dimensional parameter estimation.
The method developed in this study provides a powerful tool for the construction of reference gene sets for organisms based solely on de novo transcriptome data.
This database was used for construction of reference population means, regression coefficients, and weights used in the adjustment procedure.
IN assisted in construction of expression vectors.
OF participated in construction of cDNA libraries.
GA helped in construction of CCs.
In dairy cattle, construction of a reference population started with genotyping progeny tested bulls, merely because accurate EBV based on progeny testing were available for these bulls [ 3].
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