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Such a general approach should provide new opportunities for the understanding of molecular recognition processes in complex solutions.
The glucose biosensor retained its function in complex solutions that contained realistic concentrations of protein and potential interfering agents found in blood serum.
Thus, specific binding of the target protein of interest should be enabled even in complex solutions such as e.g., biotechnologically relevant cell lysates.
However, to facilitate a dynamic screening for oligomers also in complex solutions in high excess of monomeric species, it is equally important to have a fast on- and off-rate at each single binding site.
Lewis et al. (8) reported a "fast metabolite quantification" (FMQ) protocol to address this complication that uses rapidly acquired (12 min) 2D H C HSQC experiments to estimate the molar concentration of metabolites in complex solutions from standards at known concentrations.
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The degree of pDNA protection by CO3Ap encapsulation was compared with the naked pDNA in complex solution and PEI/pDNA complex formulation.
The measured values were not corrected for ionic strength effects in these complex solutions.
The final concentration of EDTA in the ternary complex solutions was 50 mM, and that of ATP was 5 mM.
Thus, this process can hint at composing complex solutions in problem posing by the undergraduates.
For that reason, a novel technique involving immersion in a complex solution is proposed as an alternative to traditional processing.
Axial Green's function method (AGM) is developed for the simulation of Stokes flow in geometrically complex solution domains.
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