Total RNA isolation was conducted following the manufacturer's specifications in combination with the RNeasy Mini kit (Qiagen) for RNA purification after DNase I treatment.
RNA from feces samples was extracted using TRizol in combination with the PureLink RNA Mini Kit (Ambion®, Life Technologies, Carlsbad, California, US) following the protocol by the manufacturer.
Briefly, the bacterial cell culture was stabilized with the Protect Bacteria Reagent (Ambion), and total RNA was extracted with the RNeasy Mini System (Qiagen) in combination with the RNase-Free DNase Set (Qiagen) according to the manufacturer's instructions.
RNA was extracted using the RNeasy Mini Kit (Qiagen, Doncaster, Australia) in combination with the RNase‐free DNase kit (Qiagen) carried out according to the manufacturer's instructions.
The Mini Asymmetric Radial Keratotomy can also be used in combination with the cross-linking (also known as CXL, CCR, CCL and KXL), parasurgical treatment for keratoconus.
Total RNA was isolated from allantoic fluid with Trizol LS (Invitrogen, Darmstadt, Germany) in combination with RNeasy Mini spin columns (Qiagen, Hilden, Germany).
Total RNA from healthy specimens and 119 neoplastic specimens were extracted using Trizol® according to the manufacturer's instructions and in combination with Qiagen RNAeasy Mini Kit (Qiagen).
Total RNA was extracted by using the RNeasy Mini Kit (Qiagen) in combination with QIAshredder (Qiagen) mini spin columns.
Patients were excluded if they met one of the following criteria: if the MINI indicated a major depression in combination with a score above 18 (indicating a severe depression) on the HDRS, if the MINI indicated suicidal risk, or if the MINI indicated no depression at all (Table 1).
For each channel, we used an AC-coupled, differential photoreceiver with 1 GHz analog bandwidth (Wieserlabs WL-BPD1GA) in combination with a low-pass filter (Mini-Circuits BLP-450 + ).
