Stepwise genetic engineering of cysteine-producing strains has been demonstrated in P. ananatis, where the core factors related to cysteine production were modulated in combination, including feedback-insensitive mutant alleles of SAT and 3-PGDH, efflux pumps, and the major CD (Takumi et al. 2017).
We also tested the effects of multiple factors in combination, including BMP2/7/11/Activin/GDNF, BMP2/5/7 and BMP2/NGF/NT3/BDNF.
To investigate the effect of cytokines on VEGFR-2 expression in pCSCs, the cells were cultured in R10F for 3 days in the absence or presence of cytokines alone or in combination, including recombinant murine GM-CSF, Flt3 ligand (FL), IL-3, IL-4, IL-6, IL-7 and IL-13.
We used a linear model to correct for several patient and tumor covariates, individually as well as in combination, including patient age, sex, tumor stage, and tumor site.
Multiple search terms were used in combination including recall period, reference period, self-report, test-retest, sex, drug use, HIV risk, and reliability.
Other efficacy parameters observed in this study were also comparable with previous studies using gemcitabine alone or in combination, including percent of patients with PR or SD and duration of response [ 3, 4, 6, 11, 29, 30].
Other useful findings (when used in combination) include sunken eyes, decreased activity, a lack of tears, and a dry mouth.
Commonly-used medications, often in combination, include psychostimulants, antipsychotics, mood stabilizers, and alpha-2 agonists, which vary considerably in terms of perceived safety and efficacy.
Unstimulatcd HUVECs did not produce detectable amounts of TNF-α but IFNγ IL-β and LPS in combination included TNF-α production in a time-dependent manner.
The search terms used individually or in combination, include "sepsis", "septic shock", "septicaemia", "bacteraemia", "microarrays", "RNA-Seq", "cluster analysis", "transcription profiling", "gene expression", "LPS", "endotoxin", "inflammation", and "infection".
Such interventions, which are implemented either alone or in combination, include guideline use, education and training, organisational change, surveillance and quality improvement.
