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Cleavage data suggests that the C-terminal domain is not generally involved in cleavage, although there was a consistent, yet non-significant, increase in cleavage with the topoIIα derived proteins.
Using a dose of 2uM helenalin, it was observed that levels of cleaved caspase 3, 9 and PARP were detected at the outset of 8 h post treatment with subsequent increase in cleavage with protracted treatment times.
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The probe residue also appears to have a role in hairpin resolution as this intermediate accumulates in cleavage reactions with the M289A transposase (Figure 4E).
This is evidenced by the accumulation of nicked and hairpin products in cleavage reactions with the respective mutant transposases (Figure 4D, E ).
(A ) Incorporating two glycine residues (+GG) deep within the Spitz TM segment resulted in a +5 shift in cleavage site with GlpG in E. coli cells.
It appears, thus, that vasoinhibins emerged in tetrapods and that throughout speciation, there was an increase in cleavage sites with the highest number of cleavage sites present in primate species.
(B ) Mutating a single cysteine at position 146 to serine deep within the Spitz TM segment and far away from the cleavage site (at P8′) resulted in a +5 shift in cleavage site with Drosophila rhomboid-1 in living cells.
Still, comparing supernatants of wild-type and mutant S. aureus in cleavage assays with purified CXCR2 N-terminus convincingly showed that Staphopain A is unique among S. aureus proteases to cleave CXCR2.
In each cell line, rituximab and gemcitabine result in PARP cleavage, with additional cleavage using the combination (Fig 4).
A commonly used method of testing for the presence of a gpi anchor on a protein is in vitro cleavage with phosphatidylinositol-specific phospholipase C (PI-PLC; Fig. 2) [53].
These compounds mimic the transition state in peptide cleavage with the two phosphinic oxygens tetrahedrally orientated to the inhibitor backbone.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com