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The signals were imaged on the Odyssey infrared imaging system (LI-COR Biosciences, Lincoln, NE, USA) and then normalized by internal controls, and the values for cytokines in clear medium containing 10% FBS were background subtracted.
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Whole ONs were mounted in clearing medium before imaging.
After dehydration in a graded ethanol series and diaphaneity in clearing medium, the material was embedded in Paraffins (from HuaShenPai).
Liquid scinitaillation counting was used to determine the amount of released H-arachidonic acid in cleared medium and the total radioactivity from cell monolayers solubilised in 0.1% Triton X-100.
Agar-gel immunodiffusion (AGID) is a variant of the classical serological reaction in which a soluble antigen in a clear medium is precipitated by the addition of the specific antibody.
Place one blooming or flowering tea ball in a clear, medium to large sized glass teapot or tempered glass pitcher.
The whole optic nerves were mounted in a clearing medium before imaging.
Pitch: F 6 or G6; Tone: clear, medium-long decay.
Pitch: G6; Tone: strong, clear, medium-long sustain.
Next, seeds were sown on half-strength MS medium in clear polystyrene plates (24.5 × 24.5 cm), 25 seeds for 1 line at the middle per plate.
Briefly, E. coli strains selected were grown with M9 medium in clear bottom black 384-well plates (Costar) for 4 6 h at 37 °C to reach early exponential growth (OD600 about 0.15 to 0.3).
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