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We report here that these targeted relocations behaved as hypomorphic alleles of the distantly located gene Hoxd13 and showed that posterior Hoxd genes located in cis with the integration site were down-regulated.
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Unfortunately, our analyses could not distinguish whether the m1A58 co-exists in cis with the τm5s2U anticodon wobble base modification.
Importantly, approximately half of these genes were upregulated in cis with increased transcription from the sperm allele specifically (dark red triangles in Fig. 2c).
The conformational model thus obtained indicates that the PDPP chain is mainly formed by sequences of about four skeletal bonds in the trans conformation separated by one bond in cis, with negligible incidence of cis cis orientations.
These observations together prompted us to investigate in a comprehensive fashion the relationship between TFs and abiotic stress with the integration of cis-element annotation and comparative sequence analysis using stress responsive GO terms which aimed to identifying soybean TFs which may function in abiotic stress response.
We analyzed double-targeted cells (NSK) selected after Southern blotting (Fig. S5) and metaphase FISH using Site1 and Site2 probes to examine whether Site2 integration occurred in the Site1-integrated chromosome 14 (integration in cis) or in its homologue (integration in trans) (Fig. 3B, Table 1).
It expects 30% of tickets in the CIS will be bought in the CIS with mobile apps by the end of 2014, and even more in Europe, "which is why we are investing in mobile now".
In fact, reverse the integration.
On the other hand, it was likely that the potential for cis-dominant effects of the integration site to confound the analysis would be revealed by comparing the results from either pooled or individual cell clones with untargeted integration sites.
Overall, our results have identified cis- and trans- factors resulting in the integration of signaling responses that coordinate allele-specific FLT1 transactivation by p53 and estrogen receptors.
· Segregation of children with disabilities in special schools still dominates in cis countries, but overall in the region there is a move towards integration in mainstream schools, though progress is spotty.
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