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At D73, Th17-related cytokines TGF-β and TNFα were significantly upregulated in CII immunized mice independent of the oral P. gingivalis infection, while IL-6 levels were significantly upregulated independent of CII reactivation if mice were infected with P. gingivalis.
Comparison results indicate that the %ΔE g values in CII and CIII models (0.40 and 0.56 %, respectively) are lower than those in the other models, whereas %ΔE g values in DI and EI (22.75 and 23.31 %, respectively) are more than those in the other models.
The results show a linear increase in CII activity as function of MOI (Figure 7).
Further it is remarkable that CI residue 192 that corresponds to T426 in CII is an alanine.
The mutants S17A, R24A, L26A, R27A and R32A show a cIII− phenotype in plaque morphology and in CII activity, using the pE-GFP fluorescence assay (data not shown).
The fourth difference occurs in CII in which an additional 250 bp are found in an intergenic region in the Ames strain.
Similar(14)
We previously found [ 14] a reduced proliferative T-cell response to CII stimulation in vitro in CII-primed u-PA-/ mice compared with CII-primed C57BL/6 mice even though the antibody response to CII was similar between the strains, suggesting normal immune complex formation in u-PA-/ mice following CIA induction.
Importantly, we did not find any significant difference in CII-specific antibody levels between groups (Fig. 1C).
RORγt expression and IL-17 production were significantly higher in CII-reactive CD4+ T cells from RORγt Tg mice.
Systemic treatment with osteoprotegerin prevented joint damage induced by local IL-17 gene transfer in CII-immunized mice.
We did not find COMP antibodies at any point during the whole disease course, suggesting no involvement of immune response against COMP in CII-induced arthritis.
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