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We have found no evidence that USP14 markedly changes the amount of ISG15-modified substrates in cells (data not shown).
We found that in the presence or absence of SAP after 2 days, CD93 expression was undetectable, and that 25F9 expression was detectable in cells (data not shown).
In a separate experiment, exposing the cells to FGF2 for a shorter period of time resulted in lower insulin content in cells (data not shown).
To estimate the functional half-life of ELuc and FLuc in NIH3T3 cells, we used destabilized luciferases (dELuc and dFLuc) fused to the PEST element, as we could not precisely estimate the half-life of ELuc (>8 h) because of its high stability in cells (data not shown).
However, OsRFPH2-2-EGFP did not show any fluorescence in cells (data not shown).
However, siRNA-mediated depletion of these proteins does not affect the levels of ubiquitinated EEA1 in cells (data not shown).
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I'm at home on WiFi 98% of the time and my iPhone continuously ***** in cell data.
a, Relative guide abundance before and after olaparib and cisplatin treatment in Cov362 cells (data provided in Supplementary Tables 1 and 2).
To note, p53 is deleted in FaDu and mutated in UTSCC45 cells (data not shown).
We showed that the RPB3 and eEF1γ proteins co-immunoprecipitate when over-expressed in HeLa cells (and in SY5Y cells; data not shown).
However, there was no change in ZO-1 localization in these cells (data not shown).
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