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The attenuated Gt strain, however, is not virulent in chickens and replicates well in CEF cultures.
In LEF, both PSI and PSII are involved, while only PSI participates in CEF.
Results showed that recombinant viruses were generated and correctly expressed foreign genes in CEF, BHK-21 and 293T cells.
The very virulent infectious bursal disease virus (vvIBDV) Gx strain causes over 60% mortality in chickens but cannot replicate in CEF cultures.
In vitro growth properties of recombinant virus were also inspected and concluded that the MZC12HA/NA had the same growth kinetics in CEF cultures as its parental wild type virus GX0101.
While the substituted region in VP4 had no effect on viral replication of Gt in CEF cultures, substitution of the VP3/3′UTR region did reduce the replicative capacity of the virus.
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The results showed that any one of these three adipogenic transcription factors was sufficient to trigger the adipogenic program in CEFs, as demonstrated by accumulation of cytoplasmic lipid droplets and expression of the adipocyte marker gene (adipocyte fatty acid binding protein, A-FABP).
DF-1 cells are IFN competent [34] and plasmid transfection induces IFN in CEFs [35].
rs3885907, which was most significantly associated in CEF-treated patients, was located in an intron of ALOX5AP.
The percentage of ciliary extension in CEFs was measured in at least 20 microscopic fields.
The percentage of primary cilium extension in CEFs was measured in at least 20 microscopic fields.
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