Sentence examples for in ccma from inspiring English sources

For example, the Walker A motif replacement in CcmA does not affect the stability of the ATPase subunits.

A mutant S12-22 is constructed by Chen et al. (2010), which has a transposon insertion in ccmA gene.

An ATP-driven conformational change in CcmA, presumably via CcmB, is responsible for removing the heme from CcmC.

CcmA has been shown to hydrolyze ATP, and the replacement of a lysine residue (K40 in E. coli CcmA) in the Walker A motif abolishes its function in cytochrome c maturation.

The low reactivity of the CcmE histidine is compensated by the high affinity of CcmE for heme-bound CcmC, leading to bond formation before the release of heme from CcmC and only if the CcmC heme CcmE complex occurs (accumulation of holo-CcmE in the cases where CcmA is absent or is compromised is a natural consequence).

CcmC, with the assistance of CcmD, interacts with the heme chaperone CcmE, a membrane-anchored globular protein, and transfers heme to it in a process involving CcmAB; when CcmA is inactivated, heme remains trapped in a complex with CcmC and CcmE.

This has been confirmed by the K40D mutation in the Walker A motif of CcmA that abolishes the ATPase activity of the protein; holo-CcmE accumulates in the membrane fraction and no cytochrome c is produced.

However, when the K41D-CcmA* variant was examined, a significant decrease in the level of mature holo-CcmE* in the membrane fraction was observed (∼35% compared to wild-type levels, Figure 2B), which contrasts with the significant accumulation of holo-CcmE observed in the K40D-CcmA variant of System I.

The roles of CcmA, CcmC, CcmE, and CcmF in the heme delivery process are compared between Systems I and I*.

Finally, in order to better evaluate cytosol-to-lysosome translocation of fOS a second series of pulse chase studies were performed in the presence of the vacuolar ATPase inhibitor concanamycin A (CCMA).

Holo-CcmE* was detected in the membrane at a reproducibly lower level (∼35%) with the K41D CcmA variant than the wild-type (lane 2 upper strip).